T/NXSLX 0904-2024 The content of baicalin in the stem and leaves of Scutellaria baicalensis Georgi is determined using a standard method

Scope

Scope: This document specifies the terminology, definitions, principles, reagents, materials, instruments, equipment, sample collection and preparation, determination steps, and result calculation for the determination of baicalein in Huangqin stem and leaves in Ningxia region. This document is applicable to the determination of baicalein content in Huangqin stem and leaves in Ningxia region. The detection limit is 7.8125 to 250 μg/mL. Main technical content: High-performance liquid chromatography (HPLC) is based on classical liquid chromatography. A liquid is used as the mobile phase, and different polar solvents are pumped into a column filled with a highly efficient stationary phase with extremely fine particles. The components are separated in the column and detected by a detector, thus achieving rapid and highly sensitive detection of samples. Through this method, baicalein in Huangqin stem and leaves is detected and verified. 7. Determination steps 7.1 Sample preparation: Take Huangqin stem and leaf samples and crush them in a multifunctional crusher. Pass them through a 20-mesh sieve for storage. 7.2 Preparation of liquid solutions 7.2.1 Preparation of standard solution: Precisely weigh 5 mg of baicalein and place it in a 10 mL volumetric flask. Add methanol to the mark and obtain a 0.5 mg/mL baicalein standard solution. 7.2.2 Preparation of sample solution: Precisely weigh 0.3 g of sieved Huangqin stem and leaf powder and add 40 mL of 70% ethanol to a 150 mL round-bottomed flask. Heat and reflux at 60°C for 3 hours. After cooling, filter the solution into a 100 mL volumetric flask. Wash the container and residue with a small amount of 70% ethanol, and filter the washing solution into the same volumetric flask. Finally, add 70% ethanol to the mark and shake well. Take 1 mL and add methanol to a 10 mL volumetric flask. Filter through a 0.22 μm filter membrane to obtain the sample solution. 7.3 Sample determination: Inject 20μL of the sample solution under t

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