Standard for Determining the Total Polysaccharide Content in the Stems and Leaves of Honeysuckle T/NXSLX 0902-2024

Scope

Scope: This document specifies the terms, definitions, principles, reagents, materials, instruments, equipment, sample collection and preparation, determination steps, and result calculation for the determination of total polysaccharides in the stems and leaves of Platycodon grandiflorum in the Ningxia region. This document is applicable to the determination of total polysaccharide content in the stems and leaves of Platycodon grandiflorum in the Ningxia region. The detection limit is 20200μg. Main technical content: The phenol-sulfuric acid method involves hydrolyzing sugars into monosaccharides under the action of concentrated sulfuric acid, followed by rapid dehydration to form aldehyde derivatives. These derivatives then condense with phenol to form an orange-yellow compound with stable color. At a wavelength of 490nm and within a certain concentration range, the absorbance is linearly proportional to the polysaccharide content, allowing its determination using a spectrophotometer and quantification of the sample's polysaccharide content via a standard curve, thereby enabling the detection and verification of total polysaccharides in the stems and leaves of Platycodon grandiflorum. 7. Determination steps 7.1 Sample preparation: Crush the samples of Platycodon grandiflorum stems and leaves in a multifunctional crusher and sieve them through a 20-mesh sieve for later use. 7.2 Sample extraction: Weigh 5g of sieved Platycodon grandiflorum stems and leaves (accurate to 0.0001g) into a 250mL round-bottomed flask. Add 44% ethanol at a ratio of 1:23, sonicate at 56℃ and 100Hz (250W) for 43 minutes, extract twice, combine the filtrates, and filter through a 0.22μm filter membrane. The filtered solution serves as the extract solution for Platycodon grandiflorum. 7.3 Sample determination: Accurately pipette 50μL of the Platycodon grandiflorum extract solution into a 96-well plate, mix with 1mL of 5% phenol solution, add 5mL of concentrated sulfuric acid, mix we

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