T/GXAS 488-2023 A type of Seneca virus and typing of O, A, and Asian I foot-and-mouth disease virus differential detection using multiple RT-PCR and multiple qRT-PCR methods
T/GXAS 488-2023 A type of Seneca virus and typing of O, A, and Asian I foot-and-mouth disease virus differential detection using multiple RT-PCR and multiple qRT-PCR methods
Basic Information
Scope
Scope: This document specifies the multiple RT-PCR and multiple qRT-PCR methods for the differential detection of Type A Seneca virus and Type O, Type A, and Asian Type I foot-and-mouth disease viruses, including the principles, experimental conditions, reagents, main instruments and equipment, sample collection and processing, multiple RT-PCR detection, multiple qRT-PCR detection, negative and positive controls, and result determination, etc. This document is applicable to the detection of nucleic acids from Type A Seneca virus and Type O, Type A, and Asian Type I foot-and-mouth disease viruses. Main technical content: This document specifies the multiple RT-PCR and multiple qRT-PCR methods for the differential detection of Type A Seneca virus and Type O, Type A, and Asian Type I foot-and-mouth disease viruses, including the principles, experimental conditions, reagents, main instruments and equipment, sample collection and processing, multiple RT-PCR detection, multiple qRT-PCR detection, negative and positive controls, and result determination, etc. This document is applicable to the detection of nucleic acids from Type A Seneca virus and Type O, Type A, and Asian Type I foot-and-mouth disease viruses.