T/GXAS 408-2022 Active Group standards

T/GXAS 408-2022 Agrobacterium-mediated sugarcane genetic transformation protocol

Publish Date: 2022-12-13 Implement Date: 2022-12-19 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/GXAS 408-2022

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Agriculture, forestry

ICS Name: Plant cultivation

Publish Date: 2022-12-13

Implement Date: 2022-12-19

Pages: 12 pages

Scope

Scope: This document establishes the procedure for genetic transformation of sugarcane mediated by Agrobacterium, defines the terms and definitions involved in the genetic transformation technology of sugarcane mediated by Agrobacterium, and stipulates the requirements for equipment, tools, reagents, and culture media, as well as the operating instructions for stages such as sterile seedling cultivation, preparation of Agrobacterium solution, genetic transformation, transplantation of transgenic seedlings, and detection of target genes. This document is applicable to the operation of genetic transformation of sugarcane growth points mediated by Agrobacterium. Main technical content: This document establishes the procedure for genetic transformation of sugarcane mediated by Agrobacterium, defines the terms and definitions involved in the genetic transformation technology of sugarcane mediated by Agrobacterium, and stipulates the requirements for equipment, tools, reagents, and culture media, as well as the operating instructions for stages such as sterile seedling cultivation, preparation of Agrobacterium solution, genetic transformation, transplantation of transgenic seedlings, and detection of target genes. This document is applicable to the operation of genetic transformation of sugarcane growth points mediated by Agrobacterium.