T/CSES 80-2023 The technical specification for building DNA barcodes of freshwater organisms
T/CSES 80-2023 The technical specification for building DNA barcodes of freshwater organisms
Basic Information
Scope
Scope: This document is applicable to the construction of DNA barcodes for planktonic plants, sessile algae, aquatic vascular plants, planktonic animals, large benthic invertebrates, and fish in China's freshwater ecosystems; the construction of DNA barcodes for organisms in other ecosystems can be carried out with reference to this document. Main technical content: (1) Scope; (2) Normative reference documents; (3) Terminology and definitions; (4) Reagents; (5) Equipment and materials; (6) Methods for constructing DNA barcodes; (7) Quality control and quality assurance; (8) Waste disposal; (9) Appendix A (informative) Common DNA barcode primers and PCR amplification conditions; (10) Appendix B (normative) Principles and methods of genetic distance analysis; (11) Appendix C (informative) DNA barcode data tables