T/PCA 007-2024 Determination of pesticide residues and related chemical residues in coffee using gas chromatography-mass spectrometry

Scope

Main technical content: 4. Methodological principle: The samples are extracted with acetonitrile, and the extract is purified by a solid-phase extraction column. The analysis is conducted using a gas chromatograph-mass spectrometer and quantified with an internal standard method. 5. Materials and reagents: Unless otherwise specified, only analytical-grade reagents are used in the analysis, and all experimental water must meet the requirements of GB/T 6682 for first-class water. 5.1 Reagents 5.1.1 Acetone (CH3COCH3, CAS No.: 67-64-1): Chromatographically pure. 5.1.2 N-hexane (C6H14, CAS No.: 110-54-3): Chromatographically pure. 5.1.3 Acetonitrile (C2H3N, CAS No.: 75-05-8). 5.1.4 Sodium chloride (NaCl, CAS No.: 7647-14-5). 5.1.5 Glacial acetic acid (CH3COOH, CAS No.: 64-19-7). 5.1.6 Anhydrous sodium sulfate (Na2SO4, CAS No.: 7757-82-6): Burned at 650°C for 4 hours, stored in a desiccator, and cooled before use. 5.2 Solution preparation 5.2.1 Acetonitrile-acetic acid solution (99+1, volume ratio): Measure 10 mL of glacial acetic acid and add it to 990 mL of acetonitrile, mix well. 5.3 Standard samples Chlornitrofen (C12H6Cl3NO3, CAS No.: 1836-77-7), purity ≥99.0%; Naled (C4H7Br2Cl2O4P, CAS No.: 300-76-5), purity ≥99.0%; Haloxyfop-methyl (C16H13ClF3NO4, CAS No.: 69806-40-2), purity ≥99.0%; 7,4-dichlorophenyl benzenesulfonate (C12H8Cl2O3S, CAS No.: 97-16-5), purity ≥99.0%; Fluoronitrofen (C12H6Cl2FNO3, CAS No.: 13738-63-1), purity ≥99.0%; Kinoprene (C18H28O2, CAS No.: 42588-37-4), purity ≥99.0%; Hydroprene (C17H30O2, CAS No.: 65733-18-8), purity ≥99.0%; Heptachlorepoxide (C10H5Cl7O, CAS No.: 1024-57-3), purity ≥99.0%; or standard samples certified by national authorities and granted standard material certificates. 5.4 Standard solution preparation 5.4.1 Standard stock solution (1,000 μg/mL) 5.4.2 Mixed standard stock solution (10 μg/mL) 5.4.3 Mixed standard solution (1 μg/mL) 5.4.4 Internal standard solution: Accurately

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