T/GDFCA 104-2023 Determination of the Content of Main Active Ingredients in the Extract of Radix Glycyrrhiza Glabra by High-Performance Liquid Chromatography (HPLC)
T/GDFCA 104-2023 Determination of the Content of Main Active Ingredients in the Extract of Radix Glycyrrhiza Glabra by High-Performance Liquid Chromatography (HPLC)
Basic Information
Scope
Scope: This document describes a method for determining the content of the main active ingredients in Glycyrrhizus glabra root extract by high-performance liquid chromatography (HPLC). This document is applicable to the determination of flavonoid markers (glycyrrhizin and scutellaria flavone) and triterpenoid markers (glycyrrhizol and glycyrrhetinic acid) in Glycyrrhizus glabra root extract. Other solid and semi-solid plant extracts can be referenced for implementation. This document is not applicable to Glycyrrhizus glabra root extract with a glycyrrhizin content ≥ 50%. Main technical content: 4 Principles 5 Reagents and materials 6 Instruments and equipment 7 Determination steps 7.1 Sample pretreatment 7.2 Liquid chromatography reference conditions 7.3 Liquid chromatography determination 7.4 Blank test 8 Result calculation and expression 9 Detection limit and quantification limit 10 Precision 11 Recovery rate