T/CAICC 04-2021 Rapid Detection of Carbofuran in Vegetables and Fruits by Colloidal Gold Immunochromatography Assay

Scope

Scope: This method specifies a rapid detection method for carbofuran in vegetables and fruits using colloidal gold immunoassay. This method is applicable to the rapid determination of carbofuran in vegetables and fruits except garlic and mango; Main technical content: 3 Principles: This method adopts the principle of competitive inhibition immunoassay. After extraction, the carbofuran in the sample binds to the specific antibody labeled with colloidal gold, inhibiting the binding of the antibody and the antigen on the detection line (T line) of the test strip, resulting in a change in the color depth of the detection line. By comparing the color depth of the detection line with that of the control line (C line), the carbofuran in the sample can be qualitatively determined. 4 Reagents and materials 4.1 Reagents: Unless otherwise specified, all reagents are analytical pure, and the laboratory water should meet the requirements of Grade II water in GB/T 6682. 4.1.1 Methanol (CH3OH). 4.1.2 Extraction solution: Provided by the colloidal gold immunoassay test strip or prepared according to its instructions. 4.1.3 Reference materials: The Chinese and English names, CAS registration number, molecular formula, and relative molecular mass of the carbofuran reference material are shown in Table 1. The purity shall be ≥98%. Table 1 Chinese and English names, CAS registration number, molecular formula, and relative molecular mass of the carbofuran reference material Chinese name English name CAS registration number Molecular formula Relative molecular mass Carbofuran Carbofuran 1563-66-2 C12H15NO3 221.25 Note: Equivalent traceable substances may be used. 4.2 Solution preparation 4.2.1 Carbofuran standard stock solution (100 μg/mL): Accurately weigh 1 mg of the carbofuran reference material (4.1.3) and place it in a 10 mL volumetric flask. Dissolve it with methanol (4.1.1) and dilute to the mark. Shake well to prepare a carbofuran standard stock soluti

Development Information

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