GB 1352-1986
Replaced
T/CI 004-2022
Active
Group standards
T/CI 004-2022 The content of free and total maysin in wheat grains, and the method for its determination
T/CI 004-2022 The content of free and total maysin in wheat grains, and the method for its determination
Price:
$ 20.00
Basic Information
Standard Code:
T/CI 004-2022
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
Agricultural and forestry technology
ICS Name:
Cereals, legumes, and their products
Publish Date:
2022-02-08
Implement Date:
2022-02-08
Scope
Scope: This document specifies the terms, definitions, and indicators for free tricin, total tricin, and high tricin wheat, as well as their detection methods. This document applies to the quantitative determination of free tricin and total tricin in wheat grains. Enterprises can refer to it when formulating corporate standards, and relevant institutions can use it as a reference when selecting, approving, and applying high-tricin wheat and its products. Main Technical Content: 3.1 Free Tricin Tricin, also known as tricin, medicago genistin, or wheat flavonoid, has the chemical name 5,7,4'-trihydroxy-3',5'-dimethoxyflavonoid. Free tricin possesses strong antioxidant capabilities, regulates cardiovascular function, inhibits cancer cell growth, and delays the occurrence of spontaneous breast tumors. 3.2 Total Wheat Flavonoid Total wheat flavonoid refers to the total content of flavonoids in wheat. Flavonoids, a class of polyphenols composed of two benzene rings connected by three carbon atoms, include free tricin, flavonoid aglycones, and glycoside derivatives. Flavonoids exhibit strong antioxidant and free radical scavenging properties, as well as multiple benefits such as improving cardiovascular disease symptoms, combating cancer and tumors, inhibiting bacterial and inflammatory responses, and enhancing human immunity. 3.3 High-Tricin Wheat Wheat varieties (excluding black, purple, or blue wheat) with a natural free tricin content greater than 0.5 mg/kg (inclusive) or a total tricin content greater than 1 mg/kg (inclusive) in grain development. 4. Detection Methods 4.1 Determination of Flavonoid Content in Wheat Grains High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) 4.1.1 Principle Samples are extracted with 80% ethanol solution, concentrated by centrifugation, re-dissolved, and detected using a HPLC-MS/MS system with external standard quantification. 4.1.2 Reagents Unless otherwise specified, water should