GB 1352-1986
Replaced
T/CGTA 03-2024
Active
Group standards
T/CGTA 03-2024 Determination of γ-aminobutyric acid in germinated rice using high performance liquid chromatography-tandem mass spectrometry
T/CGTA 03-2024 Determination of γ-aminobutyric acid in germinated rice using high performance liquid chromatography-tandem mass spectrometry
Price:
$ 20.00
Basic Information
Standard Code:
T/CGTA 03-2024
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
Food processing and products
ICS Name:
Cereals, legumes, and their products
Publish Date:
2024-09-20
Implement Date:
2024-11-20
Scope
Scope: This document specifies the terms and definitions, principles, reagents and materials, instruments and equipment, sample preparation and storage, analysis steps, result calculation, precision, and other requirements for the determination of γ-aminobutyric acid in germinated brown rice by high-performance liquid chromatography-tandem mass spectrometry. This document is applicable to the determination of γ-aminobutyric acid in germinated brown rice; Main technical content: 4. Principle: The sample is extracted with formic acid aqueous solution to determine γ-aminobutyric acid by high-performance liquid chromatography-tandem mass spectrometry, and quantified by external standard method. 5. Reagents and materials: Unless otherwise specified, all reagents used are chromatographically pure. Water shall be of Class I water as specified in GB/T 6682. 5.1 Reagents 5.1.1 Methanol (CH3OH). 5.1.2 Formic acid (HCOOH). 5.2 Reagent preparation 5.2.1 0.1% formic acid aqueous solution: Accurately pipette 1.00 mL of formic acid (5.1.2) and dilute to 1000 mL with water. 5.2.2 Methanol + 0.1% formic acid aqueous solution = 1 + 9 (V + V): Take 100 mL of methanol (5.1.1) and 900 mL of 0.1% formic acid aqueous solution (5.2.1) and mix. Filter through a 0.22 μm membrane (organic phase) and store for use. 5.3 Standard sample γ-aminobutyric acid standard sample (CAS No.: 56-12-2): With a purity of ≥99% or a standard sample certified and granted a standard substance certificate by the national authority. 5.4 Standard solution preparation 5.4.1 γ-aminobutyric acid standard stock solution: Accurately weigh 10.0 mg (with an accuracy of 0.0001 g) of γ-aminobutyric acid standard sample (5.3) and dissolve in water to a volume of 10 mL, giving a concentration of 1 mg/mL. Store at 4 °C for 3 months. 5.4.2 γ-aminobutyric acid standard working solution series: Prepare a 2μg/mL intermediate standard solution by diluting the γ-aminobutyric acid standard stock solution (