GB 1352-1986
Replaced
T/CI 005-2022
Active
Group standards
T/CI 005-2022 Resistant starch content and determination methods in wheat grain of high resistance to stress
T/CI 005-2022 Resistant starch content and determination methods in wheat grain of high resistance to stress
Price:
$ 20.00
Basic Information
Standard Code:
T/CI 005-2022
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
Plant protection
ICS Name:
Cereals, legumes, and their products
Publish Date:
2022-02-08
Implement Date:
2022-02-08
Scope
Scope: This document specifies the terms, definitions, scope of application, indicators of resistant starch content, and detection methods for resistant starch and high-resistant starch wheat. This document is applicable to the quantitative determination of resistant starch content in wheat seeds and products. Enterprises may refer to it when formulating corporate standards, and relevant institutions may refer to it when breeding, evaluating, and applying high-resistant starch wheat and its products. Main technical content: 3.1 Resistant starch Resistant starch, also known as enzyme-resistant starch or digestion-resistant starch, refers to a type of functional starch that cannot be degraded into glucose by starch-related enzymes in the stomach and small intestine, which helps reduce the glycemic index (GI). It also produces short-chain fatty acids through bacterial fermentation in the gastrointestinal tract and colon, promoting intestinal motility, preventing constipation, and reducing the risk of colon cancer. 3.2 High-resistant starch wheat A type of wheat variety (line) in which the natural content of resistant starch in the grain accounts for ≥6% of the dry weight during growth and development. 4. Determination methods 4.1 Principle First, the sample is digested with pepsin solution to remove proteins. Then, α-amylase and amyloglucosidase (AMG) are used to hydrolyze the non-resistant starch in the sample into D-glucose. Ethanol is added to terminate the reaction. The supernatant solution is centrifuged, and the sediment at the bottom is resistant starch. The sediment is placed in an ice-water bath and dissolved with potassium hydroxide solution. AMG is used to hydrolyze the resistant starch into D-glucose, which reacts with glucose oxidase/peroxidase (GOPOD) reagent to form a complex. The absorbance value is measured at 510 nm, and the resistant starch content is calculated based on the proportional relationship between the absorbance value and the resis