T/SSSC 003-2024 Active

Main technical content: This standard specifies the relevant terms and definitions of immobilized microbial inoculum for plant seed carriers. I. The following terms and definitions are proposed to be defined in this standard: 1. **Immobilized microbial**: Specific microorganisms are immobilized on selected carriers, achieving high density and maintaining biological activity. Under suitable conditions, they can multiply rapidly and in large quantities. 2. **Plant seed carriers**: Comprehensively utilizing seed coating technology, plant seeds with high remediation efficiency for contaminated soils are used as carriers for immobilized microorganisms. II. This standard sets technical requirements for immobilized inoculum, as follows: 1. The number of viable bacteria in the immobilized inoculum shall be not less than 108 CFUs/g. 2. The shelf life of the inoculum shall be not less than 6 months. 3. The rate of contaminating bacteria shall not exceed 30%. 4. Transportation and storage shall avoid direct sunlight, ensure ventilation and a cool environment, with a temperature maintained between 5°C and 40°C. 5. The germination rate shall be not less than 85%. 6. The water content shall not exceed 20%. 7. The pH value shall be within an appropriate range. 8. The organic matter content shall be not less than 30%. 9. The mechanical strength shall be not less than 60%. 10. The mass transfer rate shall be not less than 50%. 11. The swelling rate shall be not less than 60%. III. This standard proposes to specify the testing methods for the above indicators: 1. Determination of effective viable bacteria: Follow the provisions of GB 20287. 2. Determination of contaminating bacteria rate: Follow the provisions of NY/T 2321. 3. Determination of water content: Follow the provisions of NY/T 2321. 4. Determination of pH value: Follow the provisions of NY/T 2321. 5. Determination of organic matter content: Follow the provisions of NY 525-2021.