T/GDCA 016-2022 Active

Scope: This document specifies the methods for evaluating the soothing effects of cosmetics using the zebrafish behavioral evaluation method and the zebrafish inflammation evaluation method. This document is applicable to the soothing effect testing of cosmetic raw materials and products that are soluble in water, can be dissolved in water under certain conditions, or can form a uniformly dispersed suspension in water; Main technical content: 1. Zebrafish behavioral analysis method 1.1 Principle 1.2 Materials and reagents 1.3 Instruments and equipment 1.4 Preparation for the experiment 1.5 Pre-test 1.6 Official experiment 1.7 Result evaluation 1.8 Conditions for the validity of the experiment 2. Zebrafish inflammation evaluation method 2.1 Principle 2.2 Materials and reagents 2.3 Instruments and equipment 2.4 Preparation for the experiment 2.5 Pre-test 2.6 Official experiment 2.7 Result evaluation 2.8 Conditions for the validity of the experiment