T/SHFCA 002-2024 Active

Main technical content: This standard is applicable to the detection of vitamins A, D, and E in special medical purpose formula foods. Fat-soluble vitamins are unstable in nature and can be affected by environmental and experimental conditions. The pretreatment of samples has the most critical impact on the accuracy of data. Fat-soluble vitamins are distributed in various forms in samples, and the pretreatment process requires removing the fat-soluble substances from the matrix. First, saponification treatment is carried out, followed by extraction and purification operations. Online solid-phase extraction-two-dimensional high-performance liquid chromatography uses the online solid-phase extraction combined with centric cutting two-dimensional liquid chromatography (online SPE-2DLC) technology. After saponification, the samples are directly injected, and the processes of enrichment, purification, and separation of target analytes are automatically completed. This method shortens the analysis time of samples, further improves the analysis efficiency of the method, and can be used for routine rapid detection of vitamins A, D, and E in special medical purpose formula foods.