T/HBESA 001-2023 Active

Scope: This document describes a magnetic particle chemiluminescence assay method for the determination of benzo[a]pyrene in food. This document is applicable to the determination of benzo[a]pyrene in grains and their products (rice, brown rice, rice (flour), wheat, wheat flour, corn, corn flour, corn grits (dregs)), meat and meat products (smoked, roasted, grilled meats), aquatic animals and their products (smoked and grilled aquatic products), milk and dairy products (buttermilk, cream, anhydrous milk), fats and their products. Main technical content: This method adopts the principle of chemiluminescence competition. Benzo[a]pyrene in the sample is extracted with n-hexane, purified by molecular imprinting cartridges, concentrated to near dryness, dissolved in methanol, and then the sample solution competes with the antigen labeled in the reagent kit for binding to the benzo[a]pyrene-specific antibody conjugated to magnetic particles, or competes with the benzo[a]pyrene-specific antibody labeled in the reagent kit for binding to magnetic particles, forming an antigen-antibody complex. After magnetic separation and washing, the corresponding relative light units (RLU) are measured by an automatic chemiluminescence analyzer. The benzo[a]pyrene content in the sample is inversely proportional to the RLU value.