T/CVDA 2-2022 Active
T/CVDA 2-2022 Animal novel coronavirus neutralizing antibody detection technology
Abstract
Scope: This standard specifies the experimental operation methods and result determination criteria for the determination of neutralizing antibodies in animal serum against the novel coronavirus. This standard is applicable to the qualitative and quantitative detection of neutralizing antibodies in animal serum against the novel coronavirus; Main technical content: This draft mainly standardizes the detection method of neutralizing antibodies against the novel coronavirus in animals. By replacing the glycoprotein gene G of the VSV genome with the spike protein gene S of 2019-nCov, and then inserting the enhanced green fluorescent protein gene EGFP, a chimeric recombinant virus VSVΔG-nCoVS-EGFP expressing EGFP is constructed. This recombinant virus possesses the S protein of 2019-nCov, which can be recognized and neutralized by 2019-nCoV-positive serum to block infection.
General Product Information
| Document Type | Group standards |
|---|---|
| Standard Status | Active |
| Standard Code | T/CVDA 2-2022 |
| CCS | Agriculture, forestry |
| ICS | Veterinary medicine |
| Implement Date | 2022-07-25 |