T/CCAA 65-2023 Determination of β-Nicotinamide Mononucleotide (NMN) Content in Food by High-Performance Liquid Chromatography (HPLC)

Scope

Scope: This document specifies a high-performance liquid chromatography method for determining the content of β-nicotinamide mononucleotide (NMN) in food. This document is applicable to the determination of NMN content in NMN raw materials and NMN-containing capsules and tablets used in cross-border products. Main technical content: 1. Scope: This document specifies a high-performance liquid chromatography method for determining the content of β-nicotinamide mononucleotide (NMN) in food. This document is applicable to the determination of NMN content in NMN raw materials and NMN-containing capsules and tablets used in cross-border products. 2. Normative reference documents: The content of the following documents is an integral part of this document through normative references. Among them, the referenced documents with a specified date are only applicable to the version corresponding to that date; for the unspecified date referenced documents, the latest version (including all amendments) is applicable to this document. GB/T 6682: Specifications and test methods for water used in analytical laboratories. 3. Terms and definitions: This document does not define any terms or definitions. 4. Principle: The sample is dissolved in a 50% methanol aqueous solution, extracted by ultrasound, then centrifuged to remove impurities and filtered through a microporous membrane. The separation is performed by hydrophilic chromatography, and the detection is carried out by an ultraviolet detector using an external standard method for quantification. 5. Reagents and materials 6. Instruments and equipment 7. Determination steps 8. Calculation and presentation of results 9. Precision 10. Quantification limit

Development Information

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