T/HBAS 014-2023 Determination of Aflatoxin B1 in Food by Magnetic Micro-Particle Chemiluminescence Method

Scope

Scope: This document describes a magnetic particle chemiluminescence assay method for aflatoxin B1 in food. This document is applicable to grains and their products (corn, corn flour (residue and flakes) and corn products, paddy, brown rice, rice, wheat, barley, other grains, wheat flour, oatmeal, other shelled grains), legumes and their products (fermented soybean products), nuts and seeds (peanut and its products, other cooked nuts and seeds), vegetable oils and their products, condiments (soy sauce, vinegar, brewed sauce), infant and toddler formula foods, and infant and toddler complementary foods for the determination of aflatoxin B1; Main technical content: This method adopts the principle of chemiluminescence competition. The aflatoxin B1 in the sample is extracted with an ethanol-water solution. After the extract is centrifuged and diluted, the sample solution competes with the antigen labeled in the reagent kit to bind to the aflatoxin B1 specific antibody connected to the magnetic particle carrier, or competes with the aflatoxin B1 specific antibody labeled in the reagent kit to bind to the antigen connected to the magnetic particle carrier, forming an antigen-antibody complex. After magnetic separation and washing, the corresponding relative light unit value (RLU) is measured by an automatic chemiluminescence analyzer. The content of aflatoxin B1 in the sample is inversely proportional to the RLU value.

Development Information

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