T/GDCA 014-2022 Evaluation of Antioxidant Activity of Cosmetics using Cell-based Assays
T/GDCA 014-2022 Evaluation of Antioxidant Activity of Cosmetics using Cell-based Assays
Basic Information
Scope
Scope: This document specifies the methods and procedures for evaluating the antioxidant activity of cosmetics and their raw materials using cell systems. This document is applicable to the evaluation of the antioxidant activity of cosmetics (water-based products, emulsions, essences, etc.) and cosmetic raw materials, including natural extracts; Main technical content: 1. Evaluation methods 1.1 Cell antioxidant activity test (CAA) 1.1.1 Method principle 1.1.2 Reagents and materials 1.1.3 Instruments 1.1.4 Sample preparation 1.1.5 Cell culture 1.1.6 Cell inoculation 1.1.7 Evaluation system 1.1.8 Calculation of cell antioxidant CAA value 1.2 Inhibitory capacity of ROS in cells induced by photoaging 1.2.1 Method principle 1.2.2 Reagents and materials 1.2.3 Instruments 1.2.4 Sample preparation 1.2.5 Cell culture 1.2.6 Cell inoculation 1.2.7 Evaluation system 1.2.8 Calculation of ROS inhibitory rate 1.3 Inhibitory capacity of ROS in cells induced by hydrogen peroxide 1.3.1 Method principle 1.3.2 Reagents and materials 1.3.3 Instruments 1.3.4 Sample preparation 1.3.5 Cell culture 1.3.6 Cell inoculation 1.3.7 Evaluation system 1.3.8 Calculation of ROS inhibitory rate