T/BSGC 016-2024 Active Group standards

T/BSGC 016-2024 The determination of total flavonoids in bamboo leaves

Publish Date: 2024-06-20 Implement Date: 2024-06-30 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/BSGC 016-2024

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: -

ICS Name: Chemical analysis

Publish Date: 2024-06-20

Implement Date: 2024-06-30

Scope

Main technical content: Accurately pipette 0.0 mL, 0.5 mL, 0.8 mL, 1.0 mL, 1.3 mL, 1.5 mL, 1.8 mL, and 2.0 mL of rutin standard solution (4.2.4) into 10 mL colorimetric tubes, add 80% ethanol solution to a total volume of 4 mL, add 0.5 mL of sodium nitrite solution, shake well, and incubate for 8 minutes; then add 0.5 mL of aluminum chloride solution, shake well, and incubate for 10 minutes; add 4.0 mL of sodium hydroxide solution, and make up to the mark with 80% ethanol solution, shake well, and incubate for 10 minutes. Prepare standard series solutions with concentrations of 0 mg/L, 0.05 mg/L, 0.08 mg/L, 1 mg/L, 1.3 mg/L, 1.5 mg/L, 1.8 mg/L, and 2.0 mg/L. Use the reagent blank as the blank, and determine the absorbance value at 510 nm. Plot the standard curve with concentration as the horizontal axis and absorbance as the vertical axis. Pipette 1.0 mL of the sample solution into a 10 mL colorimetric tube, add 80% ethanol solution to a total volume of 4 mL, add 0.5 mL of sodium nitrite solution, shake well, and incubate for 8 minutes; then add 0.5 mL of aluminum chloride solution, shake well, and incubate for 10 minutes; add 4.0 mL of sodium hydroxide solution, and make up to the mark with 80% ethanol solution, shake well, and incubate for 10 minutes. Determine the absorbance value at 510 nm, and calculate the concentration of total flavonoids in the sample according to the standard curve.