T/CI 070-2022 Aerosol suspension of grasshopper microsporidium

Scope

Scope: This document specifies the product quality and inspection methods, marking, labeling, packaging, transportation, and storage requirements for water-suspended formulations of locust microsporidia. This document applies to water-suspended formulations of locust microsporidia prepared from proliferated locust microsporidia; Main technical content: Locust microsporidia (Nosema locustae) belongs to the Microsporidia order of the Protozoa phylum and is a specialized parasitic protozoan of orthopteran insects such as locusts. It is a single-celled eukaryotic organism and exists in nature as single-celled spores. The spores measure 5.5 × 3.2 micrometers and are elliptical in shape. Locust microsporidia spores are insoluble in water and remain in suspension in aqueous solutions. 3.2 Locust Microsporidia Water Suspension Formulation (Nosema locustae water suspension formulation) is a water-suspended formulation of locust microsporidia spores, which contains active spores, dispersants, thickeners, and water. 4. General Technical Requirements 4.1 Appearance: The product is a light yellow liquid containing fine particles. 4.2 Spore content: The number of viable effective spores per milliliter of solution shall be no less than 2 × 107. 4.3 Purity: 100% can pass through a 100-mesh sieve; impurities (insect tissue fragments and other debris) shall not exceed 3% of the volume of the concentrated solution; and the concentrated solution shall not contain other locust-pathogenic microorganisms. 4.4 The pathogenicity of locust microsporidia shall be determined using third-instar locusts as test insects. The LD50 of the preparation shall be ≤ 1.88 × 103 spores/head, and the product potency shall be ≥ 90%. 4.5 The shelf life of the preparation at -10°C can be preserved for more than 3 years. After 3 years, the spore viability will decrease correspondingly with storage time. At room temperature, the product's viability will decrease by no more than 10% within 1 year of storage. 5.

Development Information

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