T/ZJBX 10-2021 The determination of the proportion of protein hydrolyzates with a molecular weight less than 1000 u in protein feed
T/ZJBX 10-2021 The determination of the proportion of protein hydrolyzates with a molecular weight less than 1000 u in protein feed
Basic Information
Scope
Scope: This document describes a method for determining the proportion of protein hydrolysates with a molecular weight of less than 1000 u in protein feed using high-performance gel filtration chromatography. This document is applicable to the determination of protein hydrolysates in protein feed produced by fermentation or enzymatic hydrolysis. Main technical content: This document describes a method for determining the proportion of protein hydrolysates with a molecular weight of less than 1000 u in protein feed using high-performance gel filtration chromatography, including the principle, reagents, instruments and equipment, chromatographic conditions, preparation of molecular weight calibration curves, sample preparation, experimental steps, and calculations.