T/CCAA 41-2022 Qualitative Detection of Quinoa-Containing Components in Miscellaneous Grain Products Using Real-Time Fluorescent Polymerase Chain Reaction (RT-PCR) Method

Scope

Scope: This document describes a method for the qualitative detection of quinoa components in mixed grain products using real-time fluorescence PCR. This document is applicable to the qualitative detection of quinoa components, including white quinoa, black quinoa, and red quinoa, in mixed grain products. The detection limit (LOD) specified in this document is 0.01% (by mass ratio). Main technical content: Foreword 1 Scope 2 Normative reference documents 3 Terms, definitions, and abbreviations 3.1 Terms and definitions 3.2 Abbreviations 4 Principles 5 Primers and probes for detection 6 Reagents and materials 7 Instruments and equipment 8 Detection steps 8.1 DNA extraction 8.2 Determination of DNA concentration and purity 8.3 Real-time fluorescence PCR detection 9 Quality control 10 Result determination and expression 11 Result reporting 12 Anti-contamination measures Appendix A (Normative) Reagents and their preparation Appendix B (Informative) Reference sequence of the gene amplification target for chickpea components

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