T/SHSOT 002-2022 Chemical substance Rare Gobiidae Embryo Acute Toxicity Test
T/SHSOT 002-2022 Chemical substance Rare Gobiidae Embryo Acute Toxicity Test
Basic Information
Scope
Scope: This document specifies the information required for the test objects, the test principles, the instruments and equipment, the test system, the test procedures, quality control, and data and reports for the acute toxicity test of chemicals on the embryos of Gobiocypris rarus. This document is applicable to testing the acute toxicity effects of chemicals on the embryos of Gobiocypris rarus. Main technical content: Expose newly fertilized embryos of Gobiocypris rarus to aqueous solutions of test objects at a certain concentration range, and conduct the test in a static test, a semi-static test, or a running water test system. Observe the embryos for 96 hours, and record the observation endpoints such as egg coagulation, segment loss, unseparated tail, and no heartbeat every 24 hours. Based on any one of the four death characteristics observed, determine the median lethal concentration (LC50).