T/CBJ 1110-2025 Active Group standards

T/CBJ 1110-2025 Evaluation method for motor behavior post?consumption of alcoholic beverages based on the open field test

Publish Date: 2025-09-15 Implement Date: 2025-10-15 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/CBJ 1110-2025

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Hormones, family planning, anti-tumor drugs, and immunosuppressive drugs

ICS Name: Alcoholic beverages

Publish Date: 2025-09-15

Implement Date: 2025-10-15

Scope

This document specifies the principles, reagents and materials, experimental procedures, and post-drinking behavioral evaluation results of the evaluation method of post-drinking sports behavior of beverage alcohol based on the open-field test. This document is applicable to the evaluation of post-drinking sports behavior of the same type of beverage alcohol and beverage alcohol with the same alcohol content (within 1% vol), and rats and mice after drinking. To ensure the operability of the experimental method, the experimental groups are divided based on alcohol content. Principles After ingesting a moderate amount of alcohol, the body gradually stimulates the brain to shut down inhibitory functions and promote blood vessel dilation, making the animal appear more active in a slightly intoxicated state. However, excessive alcohol intake exceeding the brain's tolerance threshold can lead to brain suspension, reduced responsiveness, or even stupor. Therefore, after the model animals (rats and mice) ingest alcoholic beverages at a prescribed dose, the animals are placed in an open-field device for fine behavioral analysis at t0 (before alcohol ingestion) and t (after alcohol ingestion). High-frequency cameras and behavioral analysis software Anymaze are used to record and analyze the animals' free movement trajectories for 90 seconds at these time points in real time (as shown in Figure 1). When the animals move in a circular path around the periphery, it indicates that the animals exhibit normal behavior under the influence of alcohol, with a slight sense of excitement and relaxation (as shown in Figure 1a). When the animals' behavioral trajectories are disordered, it indicates that the animals exhibit moderate inhibition of behavior under the influence of alcohol, with disordered movement trajectories and occasional pauses (as shown in Figure 1b, with yellow dots on the figure). When the animals' behavioral trajectories are essentially stagnant, it indicates th