T/CCAA 76-2024 Waterborne norovirus detection method: Real-time RT-PCR method

Scope

Scope: This document specifies the real-time fluorescent RT-PCR detection method for norovirus in water bodies. This document is applicable to the qualitative detection of norovirus in water bodies, including source water, packaged drinking water, water used in food processing, aquaculture water, seawater, sewage from medical institutions, and groundwater, etc. Main technical content: - Preface - II. Scope - III. Normative reference documents - IV. Terms and definitions - V. Equipment and material requirements - VI. Reagents - VII. Operating procedures - 26.1 Sample collection and processing - 26.1.1 Membrane filtration method - 26.1.2 Automatic water sampling and microbial enrichment system method - 36.2 Virus extraction and purification - 36.2.1 Virus lysis - 36.2.2 Viral RNA extraction - 36.2.3 Viral RNA purification - 36.3 Real-time fluorescent RT-PCR detection - 36.4 Quality control - 36.4.1 Blank control - 36.4.2 Negative control - 36.4.3 Positive control - 46.4.4 Process control virus - 46.4.5 External amplification control - 47 Results and reporting - 47.1 Determination of test validity - 47.2 Result determination - 47.3 Result reporting - 48 Biosafety measures - Appendix A (informative) Removal of RNase and preparation of RNase-free solution - Appendix B (normative) Real-time fluorescent RT-PCR primers and probes - Appendix C (informative) Real-time fluorescent RT-PCR reaction system and parameters - Appendix D (informative) Process control virus Escherichia coli phage MS2 culture and primers and probes - Appendix E (informative) External amplification control RNA preparation - Appendix F (informative) Automatic water sampling and microbial enrichment system - References

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