GB/T 46929-2025 Determination of bile acids in feeds
GB/T 46929-2025 Determination of bile acids in feeds
Basic Information
Scope
This document describes the methods for determining bile acids in feed using liquid chromatography-tandem mass spectrometry and high-performance liquid chromatography.
The liquid chromatography-tandem mass spectrometry method in this document is suitable for determining bile acids (porcine bile acid, porcine deoxycholic acid, and goose deoxycholic acid) in compound feed, concentrated feed, and supplementary feed. The high-performance liquid chromatography method is suitable for determining bile acids (porcine bile acid, porcine deoxycholic acid, and goose deoxycholic acid) in additive premix feed and mixed feed additives.
The quantitative limits for porcine bile acid, porcine deoxycholic acid, and goose deoxycholic acid determined by liquid chromatography-tandem mass spectrometry in this document are all 0.10 mg/kg. The quantitative limits for additive premix feed determined by high-performance liquid chromatography are all 100 mg/kg, and the quantitative limits for mixed feed additives are all 1,000 mg/kg.