T/DACS 009-2023 Active Group standards

T/DACS 009-2023 The rapid detection method for tetracycline residue in milk using enzyme-linked immunosorbent assay (ELISA)

Publish Date: 2023-06-16 Implement Date: 2023-06-16 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/DACS 009-2023

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Forestry

ICS Name: Food industry plants and equipment

Publish Date: 2023-06-16

Implement Date: 2023-06-16

Scope

Scope: This document describes the enzyme-linked immunosorbent assay method for the determination of tetracycline, chloramphenicol, oxytetracycline, and doxycycline residues in milk. This document is applicable to the determination of tetracycline, chloramphenicol, oxytetracycline, and doxycycline residues in milk. The method's detection limit for this document is 2 μg/kg for tetracycline-class drugs. Main technical content: The sample/specimen is diluted to prepare a specimen solution. The residual tetracycline-class drugs in the specimen solution compete with the antigen on the enzyme plate for the tetracycline-class drug antibody. Under the action of the enzyme marker, the enzyme-marked antigen-antibody complex reacts with the color reagent to form a colored substrate. The absorbance value is measured using an enzyme meter, and the content of tetracycline-class drugs in the specimen is determined based on the absorbance value.