T/ACEF 227-2025 Active Group standards

T/ACEF 227-2025 Technical specification for environmental DNA monitoring of representative aquatic organisms in river basins

Publish Date: 2025-10-31 Implement Date: 2025-12-01 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/ACEF 227-2025

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: -

ICS Name: Integrated Environment and Environmental Protection

Publish Date: 2025-10-31

Implement Date: 2025-12-01

Scope

This specification stipulates the reagents and consumables, sample point layout, water sample collection, water sample filtration, DNA extraction, PCR amplification, sequencing and data analysis, and quality control for monitoring typical aquatic organisms in the Yangtze River and Yellow River basins based on environmental DNA technology. This specification is applicable to the monitoring of plankton, algae, and fish in water bodies such as rivers, lakes, and reservoirs in the Yangtze River and Yellow River basins using environmental DNA technology. 1. Technical process 2. Technical specifications 2.1 Reagents and consumables 2.2 Sample point layout 2.3 Water sample collection 2.4 Water sample filtration 2.5 DNA extraction and purification 2.6 PCR amplification 2.7 Sequencing and data analysis 2.8 Quality control 2.9 Waste disposal