T/HIFSA 0008-2024 The determination of fat in coconut milk (juice) using the Gamborg method
T/HIFSA 0008-2024 The determination of fat in coconut milk (juice) using the Gamborg method
Basic Information
Scope
Scope: This standard specifies the method for detecting fat in coconut juice (pulp). This standard is applicable to the determination of fat content in coconut juice (pulp). Main technical content: 4. Principle: Add sulfuric acid to coconut juice (pulp) to destroy the protein outer membrane on fat globules, then centrifuge to separate the fat and measure its volume. 5. Reagents and materials: Unless otherwise specified, all reagents used in this method are of analytical grade, and the water is Class III water as specified in GB/T6682. Reagents: Sulfuric acid (H2SO4). Isopentanol (C5H12O). Preparation of sulfuric acid solution (80+20): Measure 80 mL of sulfuric acid and slowly add it to 20 mL of water, then mix well. 6. Instruments and equipment: - Cream centrifuge. - Gabel's cream meter: The reading range is 0–6%, with a minimum scale value of 0.1%, as shown in Figure 1. - Commercially available domestic 10.75 mL single-standard milk suction pipettes. - Electric mixer.