T/CAIA SH006-2017 Real-time Fluorescence Nucleic Acid Test for Listeria monocytogenes in Food

Scope

Scope: This standard specifies the real-time fluorescence nucleic acid isothermal amplification detection method for Listeria monocytogenes in food. This standard is applicable to the detection of Listeria monocytogenes in foods such as meat products (cooked meat products, ready-to-eat raw meat products) and dairy products (milk and liquid dairy products, semi-solid dairy products, solid dairy products). Main technical content: At the same temperature, using bacterial RNA as the starting template, the target RNA is converted into a double-stranded DNA by M-MLV reverse transcriptase, and then multiple (100~1000) RNA copies are generated using T7 RNA polymerase as the template for this DNA; each RNA copy number then enters the next amplification cycle from the reverse transcription; simultaneously, the optimized probes with fluorescent markers specifically bind to these RNA copies, opening the stem-loop structure, thereby generating fluorescence. Based on the appearance time and intensity of the real-time fluorescence signal, combined with positive and negative controls, the detection results can be determined.

Development Information

Related Standards