T/SQIA 002-2022 Rapid Detection of Methiocarb, Chlorpyrifos, and Cypermethrin in Vegetables and Fruits by Colloidal Gold Immunochromatography Assay

Scope

Main technical content: This document specifies a rapid detection method for the residues of acetamiprid, chlorpyrifos, and chlorfluazuron in vegetables and fruits. This document is applicable to the rapid detection of acetamiprid, chlorpyrifos, and chlorfluazuron residues in vegetables and fruits. Note 1: Vegetables include cabbage, broccoli, Chinese kale, Chinese chives, spinach, ordinary cabbage, chrysanthemum greens, leaf lettuce, stem lettuce leaves, and Chinese cabbage. Note 2: Fruits include lemon. This method uses the principle of competitive inhibition immunoassay. After extraction, the acetamiprid, chlorpyrifos, and chlorfluazuron in the sample bind to the specific antibody labeled with colloidal gold, inhibiting the binding of the antibody and the antigen on the detection line (T line) of the test strip, resulting in a change in the color depth of the detection line (T line). By comparing the color depth of the detection line (T line) with that of the control line (C line), the qualitative analysis of the acetamiprid, chlorpyrifos, and chlorfluazuron in the sample can be carried out.

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