GB 1352-1986
Replaced
T/CI 003-2022
Active
Group standards
T/CI 003-2022 The content of phytic acid in low phytic acid wheat grains and its determination method
T/CI 003-2022 The content of phytic acid in low phytic acid wheat grains and its determination method
Price:
$ 20.00
Basic Information
Standard Code:
T/CI 003-2022
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
Integrated food and feed crops
ICS Name:
Cereals, legumes, and their products
Publish Date:
2022-02-08
Implement Date:
2022-02-08
Scope
Scope: This document specifies the terminology, definitions, scope, phytic acid content indicators, and detection methods for phytic acid and low-phytic wheat. This document is applicable to the quantitative determination of phytic acid in wheat seeds and products. Enterprises can refer to it when formulating corporate standards, and relevant institutions can use it as a reference when selecting, approving, and applying low-phytic wheat and its products. Main technical content: 3.1 Phytic acid hexahydric phosphoric acid (C6H18O24P6), also known as inositol hexaphosphoric acid, is an organic phosphorus compound widely found in plants. It forms insoluble compounds with metal ions such as calcium, iron, magnesium, and zinc. 3.2 Low-phytic wheat refers to wheat varieties (lines) with a phytic acid content lower than 5 g/kg naturally produced and accumulated in the grain during growth and development. 4. Detection methods 4.1 Principle: The sample is extracted with an acidic solution, and the purified phytic acid reacts with a mixture of ferric chloride and sulphosalicylic acid to produce a color change. The absorbance is measured at a wavelength of 500 nm using a spectrophotometer to calculate the phytic acid content in the sample. 4.2 Reagents Except where otherwise specified, water should comply with the requirements of Grade I water specified in GB/T 6682. 4.2.1 Reagents 4.2.1.1 Sodium hydroxide (NaOH). 4.2.1.2 Ferric chloride (FeCl3). 4.2.1.3 Trichloroacetic acid (C2HCl3). 4.2.1.4 Hydrochloric acid (HCl). 4.2.1.5 Sulphosalicylic acid (C7H6O6S·2H2O). 4.2.2 Solution preparation 4.2.2.1 0.7 mol/L sodium hydroxide solution: Weigh 28 g of sodium hydroxide and dissolve it in water to a total volume of 1000 mL. 4.2.2.2 15% trichloroacetic acid solution: Weigh 15 g of trichloroacetic acid and dissolve it in water to a total volume of 100 mL. 4.2.2.3 1.2% hydrochloric acid solution: Measure 36.5% concentrated hydrochloric acid 33.3 mL, add it to 966.7 mL of water in small am