T/CQAAS 008-2023 Active Group standards

T/CQAAS 008-2023 Determination of content of essential components in peppercorn aroma using gas chromatography-mass spectrometry (GC-MS) method

Publish Date: 2023-12-07 Implement Date: 2024-01-01 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/CQAAS 008-2023

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: -

ICS Name: Terminology (principles and coordination and cooperation)

Publish Date: 2023-12-07

Implement Date: 2024-01-01

Pages: 11 pages

Scope

Main Technical Content: 3.1 Terms and Definitions The following terms and definitions apply to this document. 3.1 Pepper Products Products produced by crushing, extraction, and freezing processes from pepper. These include pepper powder, pepper oil, preserved pepper, and pepper oil resin, among others, all made from pepper as the raw material. 3.2 Volatile Components of Pepper The main components of pepper's aromatic substances, including more than 70 volatile components such as linalool, limonene, α-pinen, 1R-α-pinene, hymenene, and β-guaiene. 4. Principle The volatile components in the sample are extracted by steam distillation, separated by a gas chromatograph column, and identified by a mass spectrometer detector. The relative content of volatile aromatic components is determined using a normalization method. 5. Reagents, Materials, and Instruments 5.1 Reagents 5.1.1 Methanol: chromatograph-grade. 5.1.2 Water: purified water. 5.1.3 Anhydrous sodium sulfate, analytical grade. 5.1.4 Micro-porous filter membrane: a 0.2μm pore size organic phase filter membrane. 5.2 Instruments 5.2.1 Gas chromatograph-triple quadrupole mass spectrometer: GCMS-TQ8050 from SHIMADZU Japan. 5.2.2 Chromatograph column: Agilent DB-624 (1.80μm×30m×0.320mm). 5.2.3 Volatile oil extractor: 5ml measurement grade, accuracy 0.05ml. 5.2.4 Analytical balance: sensitivity 0.0001g. 5.2.5 High-speed crusher: speed ≥10000rpm. 5.2.6 High-speed centrifuge: ≥4500rpm. 6. Instrument Reference Conditions 6.1 GC-MS Chromatographic Conditions A medium-polarity capillary column (Agilent DB-624, 1.80μm×30m×0.320mm) is used for chromatography. The chromatographic conditions are as follows: 6.2 GC-MS Mass Spectrometry Conditions The Shimadzu LC-MS8050 system is used to identify and confirm the substances separated by chromatography. All data collection and processing steps are carried out using the Labsolution workstation. 7. Experimental Steps 7.1 Sample Pr