T/CCAA 42-2022 Active Group standards

T/CCAA 42-2022 The qualitative detection method for coix seeding-derived components in mixed grain products using real-time fluorescence PCR

T/CCAA 42-2022 The qualitative detection method for coix seeding-derived components in mixed grain products using real-time fluorescence PCR

Publish Date: 2022-08-08 Implement Date: 2022-08-08 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us
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Basic Information

Standard Code: T/CCAA 42-2022
Standard Type: Group standards
Standard Status: Active
is_force_gb: no
CCS Name: -
ICS Name: Product certification, institutional certification, and conformity assessment
Publish Date: 2022-08-08
Implement Date: 2022-08-08

Scope

Scope: This document describes the method for qualitatively detecting coix-derived components in mixed grain products using real-time fluorescent PCR. This document is applicable to the qualitative detection of coix-derived components in mixed grain products, including Chinese cultivated coix. The detection limit (LOD) specified in this document is 0.01% (by mass ratio). Main technical content: Foreword 1 Scope 2 Normative reference documents 3 Terms, definitions, and abbreviations 3.1 Terms and definitions 3.2 Abbreviations 4 Principle 5 Primers and probes for detection 6 Reagents and materials 7 Instruments and equipment 8 Detection steps 8.1 DNA extraction 8.2 Determination of DNA concentration and purity 8.3 Real-time fluorescent PCR detection 9 Quality control 10 Result determination and expression 11 Result reporting 12 Anti-contamination measures Appendix A (Normative) Reagents and their preparation Appendix B (Informative) Reference sequences of gene amplification targets for coix-derived components

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