T/GXAS 755-2024 Marine organisms, steroidal hormones, determination, high performance liquid chromatography-tandem mass spectrometry

Scope

Scope: This document specifies the high-performance liquid chromatography-tandem mass spectrometry method for the determination of steroid hormones in marine organisms. This document is applicable to the determination of 15 types of steroid hormones in marine organisms, including androgens (nandrolone, testosterone, methyltestosterone, androsterone), progestogens (progesterone, norgestrel, medroxyprogesterone, mifepristone), glucocorticoids (cortisone, hydrocortisone, dexamethasone), and estrogens (estrone, 17-α-estradiol, 17β-estradiol, estriol). Other steroid hormones can be referenced for determination after validation of their applicability. When the sample quantity is 2.00 g, the concentrated and diluted volume is 1.0 mL, and the injection volume is 5.0 μL, the method's detection limit is 0.5–1.9 μg/kg, and the determination limit is 2.0–7.6 μg/kg, in accordance with Appendix A. Main technical content: This document specifies the high-performance liquid chromatography-tandem mass spectrometry method for the determination of steroid hormones in marine organisms. This document is applicable to the determination of 15 types of steroid hormones in marine organisms, including androgens (nandrolone, testosterone, methyltestosterone, androsterone), progestogens (progesterone, norgestrel, medroxyprogesterone, mifepristone), glucocorticoids (cortisone, hydrocortisone, dexamethasone), and estrogens (estrone, 17-α-estradiol, 17β-estradiol, estriol). Other steroid hormones can be referenced for determination after validation of their applicability. When the sample quantity is 2.00 g, the concentrated and diluted volume is 1.0 mL, and the injection volume is 5.0 μL, the method's detection limit is 0.5–1.9 μg/kg, and the determination limit is 2.0–7.6 μg/kg, in accordance with Appendix A.

Development Information

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