T/CSBM 0052-2024 The method for monitoring biodegradable magnesium metal degradation based on fluorescence probe

Scope

Scope: This document specifies the experimental design, methods, evaluation of results, and reporting for monitoring the biodegradation of magnesium-based metal products using fluorescent probes. This document is applicable to the qualitative and quantitative in vitro evaluation of the full degradation process of biodegradable magnesium-based metal products containing hydroxide magnesium, using fluorescent probes to monitor the degradation products. Main technical content: 3. Terms and definitions The following terms and definitions are applicable to this document: 3.1 Degradation: The decomposition of materials. [Source: GB/T 16886.9-2022, 3.1] 3.2 Degradation product: Particles or chemical substances generated by the chemical decomposition of the original material. [Source: GB/T 16886.9-2022, 3.7] 3.3 Corrosion: The erosion of metal materials caused by chemical or electrochemical reactions. [Source: GB/T 16886.9-2022, 3.4] 3.4 Fluorescent probe: A class of fluorescent molecules whose fluorescent properties can change sensitively with the nature of the environment. 4. Experimental design 4.1 Overview The fluorescent probe 3-(1-((4- (3-bromopropoxy) phenyl)-4-cyano-9-oxo-9H-indeno[2,1-c]pyridin-3-yl)sulfinyl)propanoic acid (PSPA), prepared according to Appendix A, will produce bright green fluorescence when combined with the main degradation product of medical magnesium alloy products, hydroxide magnesium (Mg(OH)2), under the excitation of a specific light source. The corrosion of medical magnesium alloy products can be monitored in situ using fluorescence microscopy or UV lamps, and the results can be determined by the presence and intensity of fluorescence. 4.2 Sample preparation 4.2.1 General requirements The physical properties of the samples (such as shape, surface roughness, etc.) can affect the degradation performance of the materials, and each sample should undergo the same processing and cleaning process. 4.2.2 Sample shape The samples should be round or s

Development Information

Related Standards