T/SVEFA 02011-2024 The standard for detecting the residue of seven pesticides including imidacloprid in vegetables using colloidal gold immunochromatography method

Scope

Scope: This document specifies the rapid detection method of colloidal gold immunoassay for seven pesticide residues, including cypermethrin, imidacloprid, thiamethoxam, multi-fungicide, chlorothalonil, naled, and chlorpyrifos, in vegetables. This document is applicable to the rapid qualitative determination of seven pesticides, including cypermethrin, in vegetables. Main technical content: The main content of this standard is proposed based on extensive literature review and the actual situation of rapid detection of pesticide residues in vegetables in Shanghai. The arguments are described as follows: 1. Scope: According to the actual pests and diseases and pesticide application situations in leaf vegetables, seven risky pesticides were selected for rapid detection by colloidal gold immunoassay. This standard is applicable to the rapid qualitative determination of seven pesticide residues, including cypermethrin, in vegetables. 2. Normative reference documents: According to the national standards for food safety and the requirements for water used in analytical laboratories, relevant current standards of national and industry on the maximum residue limits of pesticides in food, the specifications of water used in analytical laboratories, and testing methods are referenced. 3. Detection principle: The competitive immunoassay principle is adopted. After extraction, the target pesticides in the sample combine with the specific antibody labeled with colloidal gold to inhibit the binding of the antibody and the antigen on the detection line (T line), resulting in a color change of the T line. By visual observation (or with the help of a colloidal gold reader), the color depth of the T line in the test area of the test strip is compared with that of the control line (C line) to determine the pesticide residues in the sample. 4. Detection requirements: According to the actual pest and disease occurrence and pesticide application situations in Shanghai, the rapid detecti

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