T/FJHX 0004-2023 Determination of neutral triterpenoids and ergosterol in Ginseng extract by high performance liquid chromatography (HPLC) method
T/FJHX 0004-2023 Determination of neutral triterpenoids and ergosterol in Ginseng extract by high performance liquid chromatography (HPLC) method
Basic Information
Scope
This document specifies the high-performance liquid chromatography method for determining the content of neutral triterpenes and ergosterol in lingzhi extract. This document is applicable to the dried fruiting bodies of Ganoderma lucidum (Leyss.ex Fr. ) Karst., Ganoderma sinense Zhao, Xu et Zhang, or Ganoderma tsugae, which belong to the Polyporaceae family of fungi. It is used to determine the content of neutral triterpenes (lingzhi alcohol A, lingzhi ketone triol, and lingzhi aldehyde A) and ergosterol in lingzhi extracts prepared through processes such as extraction, concentration, drying (with or without the addition of excipients), and pulverization. In this document, the detection limit of lingzhi alcohol A is 0.227 mg/100 g, and the quantification limit is 0.758 mg/100 g.