GB/Z 170-2026 Soil quality—Measurement of enzyme activity patterns in soil samples—Using fluorogenic substrates in micro-well plates
GB/Z 170-2026 Soil quality—Measurement of enzyme activity patterns in soil samples—Using fluorogenic substrates in micro-well plates
Basic Information
Scope
This document describes a method for determining the activity of 10 soil enzymes in soil samples using fluorescent substrates, either individually or simultaneously, including arylsulfatase, α-glucosidase, β-glucosidase, cellulase, β-xylosidase, phosphodiesterase (PDE), chitinase, phosphomonoesterase (PME), leucine-aminopeptidase, and alanine-aminopeptidase.
This document is suitable for the individual or simultaneous determination of the activity of the above 10 enzymes in soil.
The enzyme activity in soil varies with seasonal changes and is influenced by soil chemistry, physical structure, and biological characteristics. When using this method to detect the harmful effects of toxic chemicals or the impact of other human activities, it is necessary to rely on the enzyme activity in control soils with similar properties to the test soils for comparative analysis, or to evaluate it through chemical exposure experiments or specific experimental treatments.