T/CAFFCI 46-2021 Active Group standards

T/CAFFCI 46-2021 Microbial testing method (qualitative) for cosmetics: ATP bioluminescent assay

Publish Date: 2021-04-30 Implement Date: 2021-05-29 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/CAFFCI 46-2021

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: -

ICS Name: Cosmetics and beauty products

Publish Date: 2021-04-30

Implement Date: 2021-05-29

Scope

Main technical content: 1. Scope This standard specifies the testing method (qualitative) for microorganisms in cosmetics—the ATP bioluminescence amplification method, including instruments and equipment, culture media and reagents, testing procedures, and result interpretation. This standard applies to the testing of microorganisms in cosmetics. 2. Normative references 3. Terms and definitions This document does not define any specific terms or definitions. 4. Abbreviations 5. Testing method 5.1 Method summary The sample is diluted to prepare a test solution. After enrichment culturing with MTAT, the ATP in microorganisms catalyzes the exogenously added ADP substrate to generate ATP, achieving ATP amplification. By adding luciferase and luciferin, the energy generated during the breakdown of ATP's high-energy bond is converted into a bioluminescence signal. The signal is detected using an automated fluorescence photometer, and the presence of microorganisms in the sample is determined based on the intensity of the bioluminescence signal. 6. Result interpretation After the test is completed, the data are processed and the results are interpreted using software.