T/FDCA 007-2022 Active Group standards

T/FDCA 007-2022 Cosmetic soothing efficacy evaluation in vitro determination of NO inflammatory mediator content lipopolysaccharide-induced macrophage RAW264.7 testing method

Publish Date: 2022-08-12 Implement Date: 2022-09-12 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/FDCA 007-2022

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: -

ICS Name: Cosmetics and beauty products

Publish Date: 2022-08-12

Implement Date: 2022-09-12

Scope

Scope: This document specifies the basic technical requirements for detecting the NO content secreted by the macrophage cell line RAW264.7 induced by bacterial lipopolysaccharide (LPS). This document is applicable to the evaluation of the soothing effects of water-soluble cosmetic raw materials and finished products. Main technical content: After macrophages are activated by LPS or interferon γ, NO is synthesized under the action of NO synthase. The process of LPS activating macrophages to release NO is accompanied by the synthesis and release of substances such as prostaglandins and tumor necrosis factor, leading to a series of immune chain reactions in the body, including anti-inflammatory and antibacterial effects. Literature indicates that various inflammatory or immune animal experiments have confirmed that NO concentration significantly increases, and inhibiting NO synthesis can improve typical inflammatory symptoms. LPS-induced RAW264.7 is a classic cell model for exploring inflammatory mechanisms. By comparing the NO content differences between the blank control group, the negative control group, and the test sample group after 24 hours of incubation, the inhibitory effect of the test sample on NO can be evaluated. The principle of NO content detection is the classic nitrite colorimetry: NO easily forms nitrite in a liquid environment. Nitrite reacts with diazo compound through a diazo reaction, and then forms a colored compound through a coupling reaction. The NO content is calculated by measuring the absorbance at 540 nm.