T/HPCIA 001-2023 Cosmetic anti-wrinkle and tightening effect - Zebra fish embryo reactive oxygen species (ROS) clearance testing method
T/HPCIA 001-2023 Cosmetic anti-wrinkle and tightening effect - Zebra fish embryo reactive oxygen species (ROS) clearance testing method
Basic Information
Scope
Scope: This document specifies the terms and definitions, raw materials, instruments and reagents, reagent preparation and preparation of test organisms, test methods, verification of test validity, result reporting, and interpretation of result relevance for the ROS removal test method in zebrafish embryos. This document provides a method for evaluating the anti-wrinkle and firming effects of cosmetics using zebrafish, which is suitable for assessing the efficacy of cosmetics that achieve anti-wrinkle and firming effects through effective ROS removal. Note: For efficacy testing of cosmetic raw materials and compound raw materials, please refer to this document. Main technical content: Foreword 1 Scope 2 Normative reference documents 3 Terms and definitions 4 Principles 5 Instruments and reagents 6 Reagent preparation and preparation of test organisms 7 Test methods 8 Verification of test validity 9 Result reporting 10 Interpretation of result relevance