T/GXAS 754-2024 Marine sediment steroid hormone determination using high performance liquid chromatography-tandem mass spectrometry

Scope

Scope: This document specifies a high-performance liquid chromatography-tandem mass spectrometry method for the determination of steroid hormones in marine sediments. This document is applicable to the determination of 15 types of steroid hormones in marine sediments, including androgens (nandrolone, testosterone, methyltestosterone, androsterone), progestogens (progesterone, norgestrel, medroxyprogesterone, mifepristone), glucocorticoids (cortisone, hydrocortisone, dexamethasone), and estrogens (estrone, 17-α-estradiol, 17β-estradiol, estriol). Other steroid hormones can be determined after suitability verification by referring to this document. When the sample amount is 5.00 g, the concentrated and diluted volume is 1.0 mL, and the injection volume is 5.0 μL, the method's detection limit is 0.5 μg/kg to 0.9 μg/kg, and the determination limit is 2.0 μg/kg to 3.6 μg/kg, according to Appendix A. Main technical content: This document specifies a high-performance liquid chromatography-tandem mass spectrometry method for the determination of steroid hormones in marine sediments. This document is applicable to the determination of 15 types of steroid hormones in marine sediments, including androgens (nandrolone, testosterone, methyltestosterone, androsterone), progestogens (progesterone, norgestrel, medroxyprogesterone, mifepristone), glucocorticoids (cortisone, hydrocortisone, dexamethasone), and estrogens (estrone, 17-α-estradiol, 17β-estradiol, estriol). Other steroid hormones can be determined after suitability verification by referring to this document. When the sample amount is 5.00 g, the concentrated and diluted volume is 1.0 mL, and the injection volume is 5.0 μL, the method's detection limit is 0.5 μg/kg to 0.9 μg/kg, and the determination limit is 2.0 μg/kg to 3.6 μg/kg, according to Appendix A.

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