T/CCAA 43-2022 Qualitative detection method for chickpea-derived components in mixed grain products using real-time fluorescence PCR

Scope

Scope: This document describes the method for qualitatively detecting chickpea-derived components in mixed grain products using real-time fluorescent PCR. This document is applicable to the qualitative detection of chickpea-derived components in mixed grain products, including the detection of cultivated chickpeas. The detection limit (LOD) of the method specified in this document is 0.01% (mass ratio). Main technical content: Foreword 1 Scope 2 Normative reference documents 3 Terms, definitions, and abbreviations 3.1 Terms and definitions 3.2 Abbreviations 4 Principles 5 Primers and probes for detection 6 Reagents and materials 7 Instruments and equipment 8 Detection steps 8.1 DNA extraction 8.2 Determination of DNA concentration and purity 8.3 Real-time fluorescent PCR detection 9 Quality control 10 Result determination and expression 11 Result reporting 12 Anti-contamination measures Appendix A (Normative) Reagents and their preparation Appendix B (Informative) Reference sequence of the gene amplification target of chickpea-derived components

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