T/GDCA 013-2022 Active Group standards

T/GDCA 013-2022 Evaluation of Antioxidant Activity of Cosmetics using Caenorhabditis elegans method

Publish Date: 2022-10-21 Implement Date: 2022-11-21 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/GDCA 013-2022

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Cosmetics

ICS Name: Cosmetics and beauty products

Publish Date: 2022-10-21

Implement Date: 2022-11-21

Scope

Scope: This document specifies the principles and procedures for evaluating the antioxidant activity of cosmetics using Caenorhabditis elegans (hereinafter referred to as nematodes). This document is applicable to the evaluation of the antioxidant activity of cosmetics (such as serums, emulsions, and creams), and cosmetic raw materials can refer to this method. This document is not applicable to cosmetics that inhibit the growth of Escherichia coli, resulting in the inability to form a smooth and thick bacterial halo on the surface of the NGM medium. Main technical content: 1. Materials and reagents 1.1 Materials 1.2 Reagents 1.3 Positive control samples 2. Experimental instruments and equipment 3. Experimental steps 3.1 Experimental preparation 3.2 Test samples 3.3 Preparation of nematode NGM 4. Experimental methods 4.1 Nematode synchronization 4.2 Pre-test 4.3 Oxidative stress survival time experiment 4.4 Criteria for judging the survival status of nematodes 5. Result evaluation 5.1 Statistical analysis 5.2 Result determination and explanation 6. Conditions for experimental validity 6.1 Sample size requirements 6.2 Quality control of synchronization experiments 6.3 Statistical differences 6.4 Safety concentration 6.5 Batch stability