T/GDCDC 030-2023 Active Group standards

T/GDCDC 030-2023 Cosmetic soothing efficacy test: In vitro measurement of inhibition of nitric oxide (NO) release by macrophages

Publish Date: 2023-02-03 Implement Date: 2023-02-03 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

Price: $ 20.00

Out of stock

Basic Information

Standard Code: T/GDCDC 030-2023

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Cosmetics

ICS Name: Cosmetics and beauty products

Publish Date: 2023-02-03

Implement Date: 2023-02-03

Scope

Scope: This document specifies the principles, materials, experimental methods, result calculation, quality control, result evaluation, and conclusions of the in vitro nitrogen monoxide (NO) release inhibition assay for macrophages. This document provides a test method for cosmetics and raw materials to inhibit the release of nitrogen monoxide (NO) induced by LPS in vitro, providing scientific support for the claims of soothing effects of cosmetics and raw materials. This document is applicable to samples that achieve soothing effects through inflammatory response pathways induced by external stimuli, but not to samples that act through other pathways, cannot be evenly dispersed in aqueous solutions, cannot penetrate the skin barrier, or have low test concentrations due to high cytotoxicity. Main technical content: 1. Principle of NO inhibition and soothing effect: Bacterial lipopolysaccharide (LPS) binds to antigen recognition receptors on macrophages, inducing the release of inflammatory mediator NO. Excessive NO can promote the release of inflammatory factors such as IL-6 and TNF-α from macrophages, which in turn can stimulate the secretion of more NO, forming a vicious cycle and exacerbating the inflammatory response. By measuring the NO inhibition rate of the sample group after administration, the inhibitory effect of the sample on NO release can be evaluated, thereby assessing whether the sample has soothing effects. 2. Principle of NO relative content test: NO is easily oxidized into nitrogen dioxide ions in the body or aqueous solutions. Under acidic conditions, nitrogen dioxide ions react with diazo salts to form diazo compounds, which further react with naphthyl vinyl diamine. The concentration of the product formed in this reaction is linearly related to the concentration of NO, and the maximum absorption peak is at 540nm. The enzyme reader is used to measure the optical density (OD) value at 540nm and calculate the relative content of NO.