T/CSBM 0050.4-2024 Active Group standards

T/CSBM 0050.4-2024 Evaluation of Wound Repair Material Efficacy - Part 4: Evaluation Method for In Vitro Microvasculogenesis Test

Publish Date: 2024-05-14 Implement Date: 2024-10-01 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/CSBM 0050.4-2024

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: -

ICS Name: Surgical instruments and materials

Publish Date: 2024-05-14

Implement Date: 2024-10-01

Scope

Scope: This document specifies the preparation of samples for in vitro evaluation of wound repair materials, the vascular formation test, and the analysis of results. This document is applicable to the evaluation of the effect of wound repair materials in promoting microvascular formation in wounds. Main technical content: 5. Vascular formation test 5.1 Principle The human vascular system is responsible for transporting nutrients and oxygen to almost all organs and tissues, and removing waste products through circulation. Vascular formation plays a significant role in the repair of tissue damage, and vascular defects can delay wound healing or even cause chronic non-healing wounds. Vascular regeneration facilitates the repair of mucosal injuries, so we used EA.HY926 cells to investigate the effect of hydrogel extracts on vascular formation. Equipment, reagents, and consumables, cell lines 5.2.1 Equipment The following equipment was used: a) CO2 incubator; b) Constant temperature water bath shaker; c) High-pressure steam sterilizer; d) Inverted fluorescence microscope; e) Cell counter; f) Constant temperature water bath shaker; g) Enzyme labeler; h) Electronic balance; i) Liquid nitrogen tank; j) Freezer centrifuge; k) T25 cell culture flask; l) 24-well plate; m) Matrigel; n) ImageJ image processing software. 5.2.2 Reagents and consumables The following reagents and consumables were used: a) MEM low-sugar medium containing 10% fetal bovine serum; b) Phosphate buffered saline (PBS); c) 0.25% trypsin. 5.2.3 Cell lines EA.HY926 cells. 5.3 Sample preparation 5.3.1 Test samples The test samples were prepared according to 4.1 and 4.2. 5.3.2 Blank control MEM low-sugar medium, 37°C, 120 rpm shaking for 24 hours. 5.4 Test procedures 5.4.1 EA.HY926 cell culture 5.4.1.1 Cell resuscitation The EA.HY926 cells were thawed in a 37°C water bath with continuous shaking until the cell freezing solution was co