T/SHSFJD 0001-2021 Sample collection and common drug testing technical specifications for municipal sewage

Scope

Scope: This document specifies the principles, reagents, instruments and materials, operating methods, and evaluation of analysis results for the sampling of domestic wastewater and the detection of common drugs. This document is applicable to the sampling techniques of domestic wastewater samples and the liquid chromatography-tandem mass spectrometry (LC-MS/MS) testing of 13 drugs and metabolites, including amphetamine (AMP), methamphetamine (MAMP), O6-monoacetylmorphine, morphine, codeine, tramadol, 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), cocaine, benzoylecgonine, ketamine, desmethylketamine, and methoxetamine, as well as the population marker cotinine. Main technical content: 4. Principles: Before laboratory analysis, domestic wastewater samples are thawed to room temperature. Then, appropriate pretreatment methods are selected to process the samples, and the determination is carried out using the multiple reaction monitoring (MRM) mode of liquid chromatography-tandem mass spectrometry (LC-MS/MS). By comparing with parallel blank samples and added samples, the retention time, mass spectral characteristic ions, and ion abundance ratios are used as qualitative monitoring and judgment criteria; the quantitative ion pair peak area is used as the basis for quantitative determination using the internal standard method. 5. Reagents, instruments, and materials: 5.1 Reagents: The test water should be Class I water in accordance with GB/T 6682. The reagents and requirements are as follows: a) Methanol: HPLC grade; b) Acetonitrile: HPLC grade; c) 98% formic acid: Superior purity; d) Hydrochloric acid: Analytical purity; e) 2mol/L hydrochloric acid (taking 2400mL as an example): Take 400mL of concentrated hydrochloric acid and add 2000mL of water, stirring while adding the hydrochloric acid to the water and mix well for storage; f) Sodium hydroxide: Analytical purity; g) 50% ammonia solution (taking 1000mL as an example): Take 100mL

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