T/CITS 218-2024 Aging biomarker: Human telomere length detection method; Real-time quantitative PCR for absolute telomere length
T/CITS 218-2024 Aging biomarker: Human telomere length detection method; Real-time quantitative PCR for absolute telomere length
Basic Information
Scope
Scope: This document specifies the principles, main equipment and materials, sample collection and processing, preparation of positive control DNA, telomere length detection, result interpretation, and method performance verification for detecting human telomere length using absolute telomere length real-time fluorescence quantitative PCR. This document is applicable to the detection of telomere length in human peripheral blood lymphocytes. Main technical content: This document specifies the principles, main equipment and materials, sample collection and processing, preparation of positive control DNA, telomere length detection, result interpretation, and method performance verification for detecting human telomere length using absolute telomere length real-time fluorescence quantitative PCR. This document is applicable to the detection of telomere length in human peripheral blood lymphocytes.